Screening of novel TMEM16C activators

초록

Lipid scrambling is a rapid process that dissipates the asymmetrical distribution of phospholipids in the plasma membrane. It is involved in various physiological functions such as blood coagulation and apoptosis. Many TMEM16 members are recognized as Ca 2+ -activated phospholipid scramblases, which transport phospholipids between the two leaflets of the plasma membrane nonspecific a lly and bidirectionally; among these, TMEM16C is abundant in the brain, especially in neuronal cells. In previous study, we investigated the scrambling acti vit y of three human TMEM16C isoforms. Among three isoforms, membrane localized TMEM16C isoforms1 and 3 could transport phosphatidylserine to the outer leaflet. After generating human TMEM16C isoform1 isoform1-overexp ressing cells, we screened new modulators for TMEM16C protein by using high content screening. We are currently testing whether these effects were observed only in TMEM16C protein or other TMEM16 fam ily members, such as TMEM16A and TMEM16F.